Ian,  I agree with Nancy. The One Step is a good stain and we use it often.  
We did shorten the procedure by microwaving 20 mls of the Bouin's for 10-15 
seconds.  Then we place the slides in the small staining container with the 
Bouin's in a 60C oven for 10-15 minutes.  Still looks great but in a few 
less minutes.  Best of Luck

Histologically yours,
Barbara H. Stancel
USDA, FSIS, OPHS, Eastern Laboratory, Pathology
RRC, 950 College Station Road
Athens, Georgia  30604
phone: (706) 546-3556
fax: (706) 546-3589



>From: Nancy E Weber <weber.326@osu.edu>
>To: Clarke Ian <clarke.ian@virgin.net>
>CC: histonet <histonet@pathology.swmed.edu>
>Subject: Re: Re- Gomoris one step trichrome
>Date: Thu, 09 Aug 2001 09:09:10 -0400
>
>Hi Ian,
>
>Here's the Gomori's One Step,  And this is the method that Victor Tobias,
>that Gayle mentioned,  would have put on the net.
>
>1. Deparaffinize and hydrate to distilled water.
>2. Place in Bouin's solution in oven at 60C for one hour.
>3. Wash will in running tap water till yellow color is gone.
>4. Rinse in distilled water.
>5. Stain in Weigert's hematoxylin for 10min.
>6.  Wash in running tap water for 10 min
>7. Place in Gomori's trichrome stain for 20 min
>8. Differentiate in 0.5% glacial acetic acid for 2 min
>9. Dehydrate, clear, and mount
>
>Muscle fibers.........................Red
>Collagen..............................Green
>Nuclei..............................Blue to Black
>
>Weigert's Hematoxylin
>      Soln A
>          Hematoxylin ..............................1.0 gm
>          95% alcohol..............................100.0 ml
>      Soln B
>          Ferric Chloride 29% aqueous.........4.0 ml
>          Distilled Water..............................95.0 ml
>          Hydrochloric Acid,  concentrated......1.0 ml
>Weigert's Working ... Mix equal parts of soln A and
>soln B.
>
>Gomori's Trichrome
>     Chromotrope 2R.............................0.6 gm
>     Light Green, SF yellowish................0.3 gm
>     Glacial acetic acid........................... 1.0 ml
>     Phosphotungstic acid........................0.8 gm
>     Distilled Water...............................100 ml
>
>Reference:  Luna, L.G.  1968, Manual of Histologic Staining Methods of the
>Armed Forces Institiute of Pathology,  (3rd Ed.) (pp 93)
>
>Nancy Weber
>Research Associate
>Veterinary Clinical Sciences
>Ohio State University
>----- Original Message -----
>From: "Clarke Ian" <clarke.ian@virgin.net>
>To: "histonet" <histonet@pathology.swmed.edu>
>Sent: Wednesday, August 08, 2001 4:39 PM
>Subject: Re- Gomoris one step trichrome
>
>
> > Hi all ,
> > I am looking for the constituents of Gomoris one step Trichrome,is it
> > comparable to the Masson Trichrome Technique?
> > Thanks in advance
> > Ian Clarke
> > Craigavon Area Hospital
> > N.Ireland
> >
> >
> >
>
>


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