RE: background in FFPE spleen

From:"Dawson, Glen" <>


Try a 10 min. pretreatment (right after deparaffinization) in 50/50
methanol/H2O2 (Hydrogen Peroxide).  I use the stuff you can buy from
walgreens.  This has helped me greatly with background in bloody tissues.

Good Luck,

Glen Dawson  BS, HT&IHC (ASCP)
Lead IHC Technologist
Milwaukee, WI

-----Original Message-----
From: Philopena, Jennifer []
Sent: Tuesday, August 14, 2001 1:57 PM
To: 'Histonet' (E-mail)
Subject: background in FFPE spleen

Hi.  I'm desperate for suggestions.  Please HELP!
I'm doing IHC on FFPE nude mouse spleen, and I'm having a BIG background
issue.  I do antigen retrieval with citrate buffer, 3% peroxidase quench for
10 minutes, protein block - 30min, rabbit polyclonal antibody - 60min,
biotin goat anti-rabbit secondary - 30min, SA-HRP - 15min, AEC-12min,
Any suggestions?
Thanks a lot.

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