RE: Tissue processing - small biopsies
From: | "Hall, Phil" <Phil.Hall@ubht.swest.nhs.uk> |
The "tubes" are filled with warmed agar. When set the pellet is placed into
a standard cassette for processing.
Phil Hall
St Michael's Hospital
BRISTOL
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From: Terry.Marshall@rgh-tr.trent.nhs.uk
[SMTP:Terry.Marshall@rgh-tr.trent.nhs.uk]
Sent: 31 July 2001 13:14
To: Terry.Coaker@nuth.northy.nhs.uk; histonet@pathology.swmed.edu
Subject: RE: Tissue processing - small biopsies
On a serious note, the best system I have come across for proper
orientation of small biopsies, is also the most laborious. However, results
were great.
It comes from the Bristol Royal Infirmary, Avon (England).
They had a series of 2ml syringes, upside down in racks. The
syringes had the nozzle end cut off, so that what remained was a tube with a
plunger at the bottom. The plunger was withdrawn a short distance and the
tube filled with (I think it was) gelatine. The tissues were then inserted
and oriented using a dissecting microscope. When the gelatine had set, the
pellet was popped out using the plunger and put into fixative.
Their slides weren't the best in the world, but their orientation
was.
Terry L Marshall
Histopathologist
Rotherham General Hospital, Yorkshire
What is the most secure, reliable, quick and cost effective way of
processing small biopsies in Tissue Tek II type cassettes?
Metal, plastic and foam inserts, filter paper, tea bag paper, agar
orientation technique - all have pros and cons.
What is really neeeded, is an agar type substitute. You place the
tisssue
on to / into this stuff in the required orientation. The tissue
sticks to
it. The stuff is resistant to solvents on a processor. You embed
it on its
flat surface. No wrapping needed, orientation correct - sorted.
Does anyone know the name of this compound ?
Thankyou
Terry Coaker
Chief Biomedical Scientist
Cellular Pathology
Royal Victoria Infirmary
Newcastle upon Tyne NE1 4LP
terry.coaker@nuth.northy.nhs.uk
0191 282 4445
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