RE: Picro-Sirius Red for Collagen Quantification?

From:"Tarpley, John" <>

Sirius red is not collagen type specific. However, it should not be read without polarization or you will falsely interpret the stain. We have used it for computerized image analysis since the collagen fibers stand out very well against the black background of other tissue elements. This makes it very easy to segment the collagen. John E. Tarpley 5-1-A Associate Scientist Amgen Inc. One Amgen Center Drive Thousand Oaks, CA 91320 These Opinions are my own and not necessarily those of my employer. -----Original Message----- From: Tracey Couse [] Sent: Tuesday, August 14, 2001 11:46 AM To: HistoNet Server Subject: Picro-Sirius Red for Collagen Quantification? I am interested in a collagen stain that will allow one of our graduate students to quantify the amount of total collagen present in coronary artery sections. I have been reviewing the archives and literature for this information. Based on my research thus far, picro-sirius red seems the best stain for this purpose. I am not interested in the birefringence properties of collagen and this stain as it is mainly collagen fiber (type I only?) birefringence that is enhanced. Is picro-sirius the best stain to use in determining the total collagen present in a given section using ordinary bright field microscopy? Any comments or suggestions would be greatly appreciated. Thanks in advance. Tracey Couse Petit Institute for Bioengineering & Biosci
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