Osteoid staining

From:Barry Rittman <brittman@mail.db.uth.tmc.edu>

I agree with Gayle re osteoid staining. You can demonstrate osteoid
using  a good H and E.  There must however be hundreds of opinions
regarding  what is a good H and E.
I think that we have to accept the fact that  H and E can be used in
different ways to demonstrate different histologic features to their
best advantage. A weak eosin will often still allow eosinophils to be
easily located and identified.
I think that one of the problems that I have encountered is the eosin
counterstain. Here in the States the most common method seems to be to
use alcoholic eosin and an alcohol differentiation.  The technique that
I was taught (admittedly in the middle ages) was to use an aqueous
solution containing eosin Y and B. After staining there was an initial
differentiation in tap water and a rapid dehydration. In my opinion this
produces a counterstain with a broader range of shades of the eosin than
can be achieved using alcoholic differentiation.  Having said that, to
show the osteoid to its best advantage as a clear pale pink, you will
have to accept a weak eosin staining in other areas such as the

<< Previous Message | Next Message >>