Gomori's one step trichrome

From:Jim Elsam <jim@hteqa.demon.co.uk>

Ian

Lillie gives "Gomori's trichrome stain" as:
Stain nuclei 5 mins with alum haematoxylin
Wash in water
Stain 5-20 mins in
	Chromotrope 2R		0.6g
	Fast green FCF		0.3g
	Phospotungstic acid	0.6g
	Glacial acetic acid	1.0ml
	Distilled water		100ml
Rinse in 0.2% acetic acid
DCM

Good luck!

Jim

>Hi all ,
>I am looking for the constituents of Gomoris one step Trichrome,is it
>comparable to the Masson Trichrome Technique?
>Thanks in advance
>Ian Clarke
>Craigavon Area Hospital
>N.Ireland

-----------------------
Jim Elsam
HTEQA Services
7 The Elms, Ringmer
Sussex, UK, BN8 5EZ
Tel: +44 (0)1273 814030
Fax: +44 (0)1273 813290
V

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