We used to use this staining method for rickettsia.  Not aware it is used
for H pylori.  I am curious about results you get, please post them.  You
might want to consider doing an immunostain for H pylori with DAB.  Very
specific.  There are some excellent methods out there, brilliant results
and it is hard to beat the Steiner with its black organisms.   

Beware, carbol fuchsin is made with phenol, considered highly toxic so work
under a hood.   


At 03:44 PM 8/1/01 -0500, you wrote:
>
>We were informed by Polyscientific that it would no longer be available. 
>It's the same with everybody. Instead, Polyscientific gave us a new H pylori 
>stain called Gimenez Technique for H Pylori. We just got it today so we 
>haven't tried it yet. It's a Carbol Fuchsin and Malachite Green combination. 
>We'll let everybody know what we think soon.
>
>We still have some Alcian Yellow that we are reserving for one pathologist 
>who is red/green color blind. :)
>
>Tara Oakes, HT (ASCP)
>
>
>>From: Bonnie McMahill <bjmcm@mail.com>
>>To: histonet@pathology.swmed.edu
>>Subject: Alcian Yellow
>>Date: Wed, 01 Aug 2001 00:15:35 +0800
>>
>>Hi!
>>
>>I guess I've totally missed all of the info going on about alcian yellow 
>>and it's unavailability through Sigma now... since we've been automated in 
>>this area. We now do not have any alcian yellow left for a backup if we 
>>need to stain manually.  Is there any available, or an alternative that 
>>will work...any help would be good, sorry to repeat this...
>>
>>Bonnie McMahill
>>Pathology Associates
>>Sacred Heart Medical Center
>>Spokane, WA
>>
>>
>>--
>>
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>
>
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>
>
Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology
Mont