Gimenez Stain procedure

From:"Charles.Embrey" <>

My procedure is so old it is titled for Camplobacter species.

Gimenez Stain

Carbol-Fuchsin (stock)
Add in order
Phenol, liquefied                   2.5ml
Ethyl Alcohol, absolute             5.0ml
Basic Fuchsin                       0.5gm
Distilled Water                    50.0ml
store at room temperature.  1 year expiration

Sodium Phosphate Buffer pH7.5 (stock)

Sodium Phosphate Monobasic                 2.7gm
Distilled Water                            100ml

Sodium Phosphate Dibasic                   2.6gm
Distilled Water                            100ml

Sodium Phosphate Buffer pH7.5 (working)

Sodium Phosphate Monobasic 2.7%       1.6ml
Sodium Phosphate Dibasic 2.6%         8.4ml

Carbol-Fuchsin (working)

Carbol Fuchsin stock                         4.0ml
Sodium Phosphate Buffer pH7.5 (working)     10.0ml
Stable 48 hours at 4 degrees Celsius.  Filter before use.


1. Deparaffinize and hydrate to distilled water.

2. Stain with Carbol-Fuchsin working solution 10 seconds.

3. Wash in distilled water, 10 seconds

4. Air dry and then dip in clearing agent, 5 dips

5. Mount with permanent mounting media

I hope this helps.  I haven't used it in years.  We just use a Giemsa stain

Charles R. Embrey Jr., PA(AAPA), HT(ASCP)
Histology Supervisor
Carle Clinic
Urbana, IL

-----Original Message-----
From: Ms.Evelyn Kaplan []
Sent: Friday, August 03, 2001 12:45 AM
To: Charles.Embrey
Subject: Re: Alcian Yellow

Good morning Charles,

I would be interested in a copy of this staining procedure as we have very
limited stocks of Alcian Yellow.

Evelyn Kaplan
Sultan Qaboos University

-----Original Message-----
From: Charles.Embrey <>
To: 'Carla Shoffeitt' <>
Cc: '' <>
Date: Friday, August 03, 2001 5:18 AM
Subject: RE: Alcian Yellow

>Gimenez is hardly a new stain.  I was using it at Children's Medical Center
>in New Orleans 18 years ago.  I should have a copy of the procedure
>somewhere in my archives if anyone is interested.
>Charles R. Embrey Jr., PA(AAPA), HT(ASCP)
>Histology Supervisor
>Carle Clinic
>Urbana, IL
>-----Original Message-----
>From: Carla Shoffeitt []
>Sent: Thursday, August 02, 2001 1:03 PM
>Subject: Re: Alcian Yellow
>  This new h.pylori stain from Poly-Scientific sounds like an AFB stain.  I
>am curoius to know more about it and how it works.  Do you have a procedure
>for it yet?
>Carla J. Shoffeitt
>Histology Supervisor
>Columbus Regional Medical Center
>Columbus, GA
>>From: Tara Miller <>
>>Subject: Re: Alcian Yellow
>>Date: Wed, 01 Aug 2001 15:44:24 -0500
>>We were informed by Polyscientific that it would no longer be available.
>>It's the same with everybody. Instead, Polyscientific gave us a new H
>>stain called Gimenez Technique for H Pylori. We just got it today so we
>>haven't tried it yet. It's a Carbol Fuchsin and Malachite Green
>>We'll let everybody know what we think soon.
>>We still have some Alcian Yellow that we are reserving for one pathologist
>>who is red/green color blind. :)
>>Tara Oakes, HT (ASCP)
>>>From: Bonnie McMahill <>
>>>Subject: Alcian Yellow
>>>Date: Wed, 01 Aug 2001 00:15:35 +0800
>>>I guess I've totally missed all of the info going on about alcian yellow
>>>and it's unavailability through Sigma now... since we've been automated
>>>this area. We now do not have any alcian yellow left for a backup if we
>>>need to stain manually.  Is there any available, or an alternative that
>>>will work...any help would be good, sorry to repeat this...
>>>Bonnie McMahill
>>>Pathology Associates
>>>Sacred Heart Medical Center
>>>Spokane, WA
>>>FREE Personalized E-mail at
>>>Talk More, Pay Less with Net2Phone Direct(R), up to 1500 minutes free!
>>Get your FREE download of MSN Explorer at http://

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