Gimenez Stain procedure
My procedure is so old it is titled for Camplobacter species.
Add in order
Phenol, liquefied 2.5ml
Ethyl Alcohol, absolute 5.0ml
Basic Fuchsin 0.5gm
Distilled Water 50.0ml
store at room temperature. 1 year expiration
Sodium Phosphate Buffer pH7.5 (stock)
Sodium Phosphate Monobasic 2.7gm
Distilled Water 100ml
Sodium Phosphate Dibasic 2.6gm
Distilled Water 100ml
Sodium Phosphate Buffer pH7.5 (working)
Sodium Phosphate Monobasic 2.7% 1.6ml
Sodium Phosphate Dibasic 2.6% 8.4ml
Carbol Fuchsin stock 4.0ml
Sodium Phosphate Buffer pH7.5 (working) 10.0ml
Stable 48 hours at 4 degrees Celsius. Filter before use.
1. Deparaffinize and hydrate to distilled water.
2. Stain with Carbol-Fuchsin working solution 10 seconds.
3. Wash in distilled water, 10 seconds
4. Air dry and then dip in clearing agent, 5 dips
5. Mount with permanent mounting media
I hope this helps. I haven't used it in years. We just use a Giemsa stain
Charles R. Embrey Jr., PA(AAPA), HT(ASCP)
From: Ms.Evelyn Kaplan [mailto:firstname.lastname@example.org]
Sent: Friday, August 03, 2001 12:45 AM
Subject: Re: Alcian Yellow
Good morning Charles,
I would be interested in a copy of this staining procedure as we have very
limited stocks of Alcian Yellow.
Sultan Qaboos University
From: Charles.Embrey <Charles.Embrey@carle.com>
To: 'Carla Shoffeitt' <email@example.com>
Cc: 'firstname.lastname@example.org' <email@example.com>
Date: Friday, August 03, 2001 5:18 AM
Subject: RE: Alcian Yellow
>Gimenez is hardly a new stain. I was using it at Children's Medical Center
>in New Orleans 18 years ago. I should have a copy of the procedure
>somewhere in my archives if anyone is interested.
>Charles R. Embrey Jr., PA(AAPA), HT(ASCP)
>From: Carla Shoffeitt [mailto:firstname.lastname@example.org]
>Sent: Thursday, August 02, 2001 1:03 PM
>To: email@example.com; firstname.lastname@example.org
>Subject: Re: Alcian Yellow
> This new h.pylori stain from Poly-Scientific sounds like an AFB stain. I
>am curoius to know more about it and how it works. Do you have a procedure
>for it yet?
>Carla J. Shoffeitt
>Columbus Regional Medical Center
>>From: Tara Miller <email@example.com>
>>Subject: Re: Alcian Yellow
>>Date: Wed, 01 Aug 2001 15:44:24 -0500
>>We were informed by Polyscientific that it would no longer be available.
>>It's the same with everybody. Instead, Polyscientific gave us a new H
>>stain called Gimenez Technique for H Pylori. We just got it today so we
>>haven't tried it yet. It's a Carbol Fuchsin and Malachite Green
>>We'll let everybody know what we think soon.
>>We still have some Alcian Yellow that we are reserving for one pathologist
>>who is red/green color blind. :)
>>Tara Oakes, HT (ASCP)
>>>From: Bonnie McMahill <firstname.lastname@example.org>
>>>Subject: Alcian Yellow
>>>Date: Wed, 01 Aug 2001 00:15:35 +0800
>>>I guess I've totally missed all of the info going on about alcian yellow
>>>and it's unavailability through Sigma now... since we've been automated
>>>this area. We now do not have any alcian yellow left for a backup if we
>>>need to stain manually. Is there any available, or an alternative that
>>>will work...any help would be good, sorry to repeat this...
>>>Sacred Heart Medical Center
>>>FREE Personalized E-mail at Mail.com
>>>Talk More, Pay Less with Net2Phone Direct(R), up to 1500 minutes free!
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