keratin staining

<< Previous Message | Next Message >>
From:Barry Rittman <brittman@mail.db.uth.tmc.edu>
To:histology <histonet@pathology.swmed.edu>
Reply-To:
Content-Type:text/plain; charset=us-ascii

> Bruce,
> There is no foolproof method that I know of for specifically identifying keratin although you can demonstrate individual components.
> Are you trying to demonstrate the number or amount fully keratinized squames in the upper layers of the skin or the presence of intermediate filaments?
> If the former then just a good H and E (easy on the E) will do the job and the stratum corneum will be easy to distinguish form the stratum granulosum.
> If you wish for a more sophisticated trichrome, then the PAS-SACPIC trichrome is time consuming but works well to show the different layers.
> If trying to determine the numbers of  layers of squames then use unfixed frozen sections, place a few drops of a pH 10-11 buffer containing a low percentage of methylene blue (0.05%) and add a coverslip. The squames will expand and the individual cells will take up the stain.
> Can also use cytokeratin staining.
> Barry
>
> Bruce Abaloz wrote:
>
> > We are hoping someone can help with this? Need to stain keratin in the foreskin of Human Penises & have so far done the Attwood's Stain for Amniotic Fluid Embolism which also demonstrates Keratin but hoping there is another stain we can do!! ANY suggestion that is "tried & true" will be most appreciative. Cheers & thanks in advance.......Bruce in OZ.
> >
> > BRUCE ABALOZ
> > HISTOLOGIST
> > DEPARTMENT of ZOOLOGY * ph: +61 3 93446282
> > The UNIVERSITY of MELBOURNE * fax: +61 3 93447909
> > Parkville, Victoria.3052 * email: b.abaloz@zoology.unimelb.edu.au
> > AUSTRALIA.
> > OPEN THE MIND;STRENGHTHEN THE BODY;FORTIFY THE SPIRIT!!
> > DANCE, LIKE NO - ONE'S WATCHING!!
> > This message is confidential;its contents do not constitute a commitment by The University Of Melbourne except where provided for in a written agreement between you and The University Of Melbourne. Any unauthorised disclosure, use or dissemination, either whole or partial, is prohibited. If you are not the intended recipient of the message, please notify the sender immediately.




<< Previous Message | Next Message >>