immunostainig
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| From: | Garry Ashton <GAshton@picr.man.ac.uk> |
| To: | "'Histonet@pathology.swmed.edu'" <Histonet@pathology.swmed.edu> |
| Reply-To: | |
| Content-Type: | text/plain; charset=iso-8859-1 |
Dear all,
Has anybody out there had any experience of a haematoxylin counterstain
being prevented from working, after immunostaining with an antibody. The
researcher presenting the problem doesn't know whether the Ab is nuclear or
cytoplasmic marker.
It cannot be fixation or necrotic tissue causing the problem as the negative
controls apparently stain fine as does her staining with other antibodies.
Any suggestions are welcome.
Garry
Paterson Institute
Wilmslow Road
Manchester
M20 9BX
UK
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