Toluidine blue stain for undecalified bone (MMA)

<< Previous Message | Next Message >>
From:Nancy Maronto <nmaronto@hotmail.com>
To:histonet@pathology.swmed.edu
Reply-To:
Content-Type:text/plain; format=flowed

I am looking for a procedure for T blue stain at pH of 8 for undecalcified 
bone in MMA.  These are thin sections (5 microns).  I tried the procedure in 
Sheehan's Histotechnology book but used a phospate buffer to reach the pH. 
This caused a problem with what I think was precipitate on the slide.  Any 
suggestions?

Thanks,
Nancy Maronto
MPI Research
Mattawan, MI
(616)668-3336
________________________________________________________________________
Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com




<< Previous Message | Next Message >>