Toluidine blue stain for undecalified bone (MMA)

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From:Nancy Maronto <>
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I am looking for a procedure for T blue stain at pH of 8 for undecalcified 
bone in MMA.  These are thin sections (5 microns).  I tried the procedure in 
Sheehan's Histotechnology book but used a phospate buffer to reach the pH. 
This caused a problem with what I think was precipitate on the slide.  Any 

Nancy Maronto
MPI Research
Mattawan, MI
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