Toluidine blue stain for undecalified bone (MMA)
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From: | Nancy Maronto <nmaronto@hotmail.com> |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; format=flowed |
I am looking for a procedure for T blue stain at pH of 8 for undecalcified
bone in MMA. These are thin sections (5 microns). I tried the procedure in
Sheehan's Histotechnology book but used a phospate buffer to reach the pH.
This caused a problem with what I think was precipitate on the slide. Any
suggestions?
Thanks,
Nancy Maronto
MPI Research
Mattawan, MI
(616)668-3336
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