Re: processor artifacts in tissue

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From:Richard Pitman <Richard.Pitman@wri-tr.wmids.nhs.uk>
To:CJones6672@aol.com, histonet@pathology.swmed.edu
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Richard Pitman FIBMS,
Head MLSO,
Dept of Histology, Cytology & Immunology,
Worcester Royal Infirmary NHS Trust

>>> <CJones6672@aol.com> 08/21/00 02:01am >>>
My Histology Lab is currently using a Leica tissue processor and have 
encountered some severe processing problems.  The stained slides have nuclear 
"ghosting" and   a washed out appearance. The problem appears to be reagent 
carry over as it usually starts occurring about 2 to 3 runs after all of the 
reagents are changed. We have had the machine serviced and checked numerous 
time without solving the problem. 
Has anyone else encountered this problem with their Leica? 

In a word, yes.

It is obviously due to your junior staff not changing the solutions properly - well, that's what Leica told me ...

I have probably exhausted the patience of this list on this subject, so will e-mail privately. Previous postings should be in the archives, for the curious.

Good luck, 




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