Re: brazilin/red nuclear stains
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From: | Geoff McAuliffe <mcauliff@UMDNJ.EDU> |
To: | "Komuves, Laszlo" <LKOMUVES@corr.com> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
> From: J. A. Kiernan [mailto:jkiernan@julian.uwo.ca]
> Sent: Monday, August 28, 2000 7:52 AM
> To: Smith, Allen
> Cc: Histonet
> Subject: Re: brazilin
>
> On Sun, 27 Aug 2000, Smith, Allen wrote:
>
> > I used to use brazilin, but I have been unable to find it lately. Where
> can I buy it?
>
> Dear Allen, Your question may be of wider interest, so I'm
> forwarding the answer to HistoNet, in the hope that someone
> knows a less expensive supplier. I could find Brazilin in only
> two catalogues. Details below.
>
> I like alum-brazilin as a red nuclear stain, but the dyestuff
> has become ridiculously expensive in the last 10 years or so.
> I've still got a bit left over from the 1970s when its price
> wasn't in any way remarkable.
>
> Brazilin. CI 74280 Natural red 24
> ICN Biomedicals Inc. Cat # 154862 US$58 for 1 gm.
> VWR Canlab Rare & Fine Chemicals. Cat # 205613 US$1776.50 for 10 gm.
> (VWR say they can supply smaller amounts!)
> The 2nd of these catalogues is more recent (2000). It seems
> to be an ICN catalog in a VWR wrapper.
>
> Brazilwood chips can be bought for back-to-nature home dyeing.
> Brazilin is obtained by evaporating an alcoholic extract, in
> just the same way that haematoxylin is made from logwood.
> Sounds easy if you have the time; I've never tried it myself.
>
> Some years ago a Histonetter in Australia reported having
> made some workable haematoxylin out of home-grown logwood,
> and there was other correspondence about this and brazilin
> around the same time. It should be in the Archives.
>
> John A. Kiernan,
> Department of Anatomy & Cell Biology,
> The University of Western Ontario,
> LONDON, Canada N6A 5C1
Dear List:
On the subject of red nuclear stains, I have found "Scarba Red" (Slidders et
al., J. Path. Bacteriol. 75:476-478, 1958) to be relatively easy to make and
use. It is a much brighter color, a red-orange, than the nuclear fast red I have
used. Certainly cheaper than Brazilin.
Geoff
--
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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