Re: blocking endogenous peroxidases

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From:Victoria Baker <vbaker60@yahoo.com>
To:Marek Malecki <mmm@ucsd.edu>
Reply-To:
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Have you tried 3% H2O2 in absolute methanol for 10
minutes?

I have used this with both human and animal tissue
samples both with IHC and in-situ protocols.  It does
work and it has been very beneficial especially when
working with polyclonal antibodies.

Sincerely,

Vikki Baker
American Health Foundation
Valhalla, New York
--- Marek Malecki <mmm@ucsd.edu> wrote:
> Would anyone have a suggestion for blocking
> endogenous peroxidases before 
> HRP labeling better than methanol/H2O2 or NaN3?
> 
> M. Malecki, M.D., Ph.D.
> Director and Principal Investigator
> 
> Molecular Imaging Laboratories
> University of California at San Diego
> address: 1500 Suite Bonner Hall, 9500 Gilman Drive,
> La Jolla, CA 92093-0368
> phone: 8588223373
> fax: 8588223715
> pager: 8586161420
> e.mail: mmm@ucsd.edu
> e.pager: 1620024619@alphapage.airtouch.com
> www site: http://mil.ucsd.edu/
> ftp site: mil1.ucsd.edu
> 
> 
> 
> 
> 
> 


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