Re: Permanent mounting media for AEC

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From:Abizar Lakdawalla <abizarl@innogenex.com>
To:Lesley Weston <lesley@interchange.ubc.ca>
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We use the Supermount fixative for our fluorescent experiments (phycoerythrin, and
some of the standard dyes) as there is no autofluorescent emission.
Abizar
www.innogenex.com
1-877-igx-info


Lesley Weston wrote:

> Do you happen to know if these mounting media autofluoresce? (I don't know what
> AEC is, so this might be a silly question.) I've been searching for a permanent
> aqueous mdium for various fluorescent stains, so if one or more of the mountants
> you mention is free of fluorescence, then I would love to know about it. Thanks.
>
> Lesley Weston.
>
> On Sun, 6 Aug 2000, Gayle Callis wrote:
>
> > You can still purchase Crystal Mount from Fisher aka Fisher Healthcare
> > catalog for clinical labs, still made by Biomeda Corp. Have used this with
> > great success for AEC and is available in larger volume (bulk) bottle and
> > sent with smaller dispensing bottle, far cheaper than individual tiny
> > bottle purchases.
> >
> > Seems this "permanent aqueous" mounting media is available from several
> > companies.  Have a sample from Biogenex which is excellent called
> > Supermount.  Dako also carries one in their catalog, name escapes me, and
> > the AquaPerm from Shandon Lipshaw.  They all work the same way.
> >
> >
> >
> >
> >
> >
> > Gayle Callis
> > Veterinary Molecular Biology
> > Montana State University
> > Bozeman MT 59717-3610
> > 406 994-4705
> > 406 994-4303
> >
> >




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