<< Previous Message | Next Message >>
From:Geoff McAuliffe <mcauliff@UMDNJ.EDU>
To:Louise Taylor <>
Content-Type:text/plain; charset=us-ascii

Louise Taylor wrote:

> Hello histonetters
> I want to ask a REALLY DUMB question: How does one accurately measure out
> Tween 20 ( pipette, measuring cylinder)? It is so  viscous that I always
> doubt that i have washed out/expelled the correct amount.

    I put 90 ml of dist. water in a 100 ml graduate, then carefully add
Trition or Tween to the 100 ml mark. Drop in a small stir bar, put it on the
mag. stirrer for a while, and you have a relatively easy to handle 10%

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

<< Previous Message | Next Message >>