Re: Internal Elastic Lamina

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From:labpath <labpath@scmc.org>
To:hooser@anatomy.iupui.edu, histonet@pathology.swmed.edu, jean.reed@pharma.Novartis.com
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>>> <jean.reed@pharma.Novartis.com> 08/29/00 12:03PM >>>

Dear Taffy,
Have you tried doing Verhoff Van Geison stain on these tissues.  This is
the stain that I do on arteries, but my tissues are in paraffin.  When I
have to do image analysis I just leave out the Van Geison part of the
stain.  With just the Verhoff the elastin only is stained and gives a nice
almost black and white stain.  I use the Elastin Stain kits from Sigma (cat
# HT 25-A).

Hope this helps,

Jean Reed
MCD Pharmacology
Novartis Pharmacology Corp.
Summit, NJ



                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 




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