I have found that several markers, including CD45, Ki67, CD3, CD79 lose 
antigenicity rather quickly if cut in advance of staining.  To ensure all 
ICH are properly controlled, we have a policy of cutting the control slides 
at the same time the patient case is cut.  It may be a bit more time 
consuming, but it has prevented having to repeat cases because the controls 
did not react properly.  It always confuses the docs if the patient case 
stains darker than the control.

Louri Caldwell



>From: Chewy71874@aol.com
>To: histonet@pathology.swmed.edu
>Subject: Re: IHC precut controls
>Date: Sat, 19 Aug 2000 03:44:11 -0400 (EDT)
>
>We cut ER & PR controls fresh each time because we have found they lose
>strength fairly quickly.  We only occasionally get orders for them.
>
>Ellen Yee
>CHF
>Sacramento
>

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