Re: IHC for Cytokeratin on Cytospins

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From:DDittus787@aol.com
To:kchapman@mail.med.upenn.edu, DAVIDDAN@aol.com, atbrooks@snet.net, histonet@pathology.swmed.edu
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Cam 5.2 works great on the Ventana , a Protease 1 digestion for 4 min, and a 
32 min incubation. I would try dilutions around 1:20 to 1:100 if you have 
enough slides,
fix the cells with formalin 10min at rm temp, then place on instrument. i 
have done this many times and it has worked great.
                                                        dana



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