Re: IHC for Cytokeratin on Cytospins
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From: | DDittus787@aol.com |
To: | kchapman@mail.med.upenn.edu, DAVIDDAN@aol.com, atbrooks@snet.net, histonet@pathology.swmed.edu |
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Cam 5.2 works great on the Ventana , a Protease 1 digestion for 4 min, and a
32 min incubation. I would try dilutions around 1:20 to 1:100 if you have
enough slides,
fix the cells with formalin 10min at rm temp, then place on instrument. i
have done this many times and it has worked great.
dana
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