Re:IHC for Cytokeratin on Cytospins
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| From: | Kenneth Chapman <kchapman@mail.med.upenn.edu> |
| To: | Dan <daviddan@aol.com>, Dana Dittus <ddittus787@aol.com>, Amos Brooks <atbrooks@snet.net>, "histonet@pathology.swmed.edu" <histonet@pathology.swmed.edu> |
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Hi Everyone,
First of all, let me thank you all for responding! The antibody which I
have been given is from BD Biosciences (Becton Dickinson).
It is Anti-Cytokeratin (Clone:CAM 5.2) directed against Cytokeratin 7
and 8.
I will be running on the Ventana Automated IHC Stainer using the Basic
DAB detection kit.
The cells have not yet been fixed and will be stored in -20 until use.
The antibody is supplied as 25 ug/ml and is monoclonal.
Any help that you can give me as far as fixation, blocking, dilutions,
etc. would be EXTREMELY appreciated.
Thanks a bunch!!
Ken Chapman
Cell Morphology Core
University of Pennsylvania
Institute for Human Gene Therapy
Philadelphia, PA 19104
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