Re: Dewaxing slides

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From:Amos Brooks <>
To:Louise Taylor <>
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    I have left slides in water overnight both before and after the staining
run. I would not advise letting them air dry after deparaffinization though.
Ideally, if you are to leave the slides out before the staining run, you should
leave them in buffer. Better safe than sorry, you may run the risk of background
staining, probably not loss of antigenicity.
Amos Brooks

Louise Taylor wrote:

> Hi histonetters
> Has anyone had any experience with loss of antigenicity in slides that have
> been dewaxed and either left soaking in water/buffer overnight or allowed to
> airdry?
> The reason I ask this is that we are moving to an automated IHC sytem in the
> near future and will be dealing with a lot of slides and a short turn around
> time.
> Any input appreciated
> Louise Taylor
> Research Laboratory
> Department of Anatomical Pathology
> South African Institute for Medical Research
> Johannesburg
> South Africa

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