From: | burch007@mc.duke.edu |
To: | cklein@mail.mdanderson.org |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
I haven't used nickel chloride, but have used cobalt. Add 200 ul of 1% cobalt chloride to 50 ml of working DAB. Develop as usual. Hope this helps. Jim Burchette Duke Immunopath