Re: DAB with Nickel procedure

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From:burch007@mc.duke.edu
To:cklein@mail.mdanderson.org
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I haven't used nickel chloride, but have used cobalt.

Add 200 ul of 1% cobalt chloride to 50 ml of working DAB. Develop as usual.

Hope this helps.

Jim Burchette
Duke Immunopath





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