Re: Breast Asp. Fluid
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From: | Patricia E Quattrone <pquattrone@juno.com> |
To: | AmyS@gmhsc.com |
Reply-To: | |
Content-Type: | text/plain |
Amy,
Our specimen collection protocol for cytology fluids is that they are
fixed with equal parts of specimen and 50% ETOH upon collection if the
specimen cannot be delivered to the Histology dept within 15minutes. We
fix our smears for at least 1/2 hour in 95% ETOH before staining. Also if
you hand stain be more gentle. If automated be sure the rate-of-flow of
rinse water is not too forceful. Use of cytospin will help to concentrate
cells in a small area. Just because the fluid is turbid doesn't mean
there are lots of cells - it just might be protein. Hope this helps
Pat Quattrone HT
Olean, NY
On Tue, 15 Aug 2000 17:33:37 -0400 Amy Self <AmyS@gmhsc.com> writes:
>
> Help! Does anyone out there know of a procedure for breast
> asp.
> fluids that will not take/wash off during staining. It seems like
> everytime
> I do a cytology on breast fluid there in nothing on the slide for
> the
> Pathologist to read. What am I doing wrong. My fixative is 70%
> alcohol
> and I use postive charged slides. I spin the fluid down and pour
> off the
> supernatent and use the button at the bottom for my cells and still
> nothing.
> Does anyone have any suggestions about anything that I could do
> different
> with these specimens?? Processing breast cyst fluids seems to be a
> process
> for me!
>
>
> Thanks,
> Amy
>
>
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