Hi,
    The staining of the tissue should be unaffected assuming the titer
has
been properly optimized. The 4 degree incubation would progress at a
slower
rate so the antibodies would have to be a be more concentrated, or
incubated
longer than at room temperature. Either way the titer should be
optimized to
get the same stain intensity.

Amos Brooks

Nima Farsinejad wrote:

> I have been reading about staining Alpha Smooth Muscle Actin in Ito Cells
> recently. I have noticed that most people incubate their primary antibody
> at 4oC overnight and some do it for 1 hour at room temperature. Does
> anyone know what differences could this change impose upon the staining of
> the tissue?
>
> Nima Farsinejad
> Research Specialist
> Digestive Diseases
> Emory University
> Atlanta, Georgia