Tora:

Humason (5th edition) recommends narcotizing echinoderms in fresh water or
sprinkling methanol on the surface of the water.  Magnesium chloride or
sulfate can be added gradually.  Then place the animal in mercuric chloride
- acetic acid......however no recipe or fixation time given.  The reference
given is Moore, AR (1962) Collecting and preserving the developmental stages
of the Pacific coast bat starfish, Patria miniata.  Ward's Bulletin 1.

Regards -
Carol
*****************
Carol B. McCollough, HT(ASCP)
Diagnostics & Histology Laboratory Manager
Maryland Department of Natural Resources
Fisheries Service
Cooperative Oxford Laboratory
904 S. Morris Street
Oxford, MD 21654


-----Original Message-----
From: Tora Bardal [mailto:Tora.Bardal@chembio.ntnu.no]
Sent: Wednesday, August 23, 2000 9:15 AM
To: histonet@Pathology.swmed.edu
Subject: sea cucumber


Hello

Does anyone happen to have a fixation protocol for Sea cucumber they can
share??

:-)
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Tora Bardal					tlf: + (47)73 59 09 38
Department of Zoology, 			fax: + (47)73 59 63 11
Norwegian University of Science and Technology (NTNU)
Brattoera Research Center
N-7491 Trondheim				Tora.Bardal@chembio.ntnu.no
Norway