Dear Richard,
Are you sure it's processing and not staining?
Ruth
Ruth A. Yaskovich
CIC, Histology Branch
National Institute of Dental and Craniofacial Research
National Institutes of Health
Phone# 301-496-1392
Fax# 301-443-3338


> ----------
> From: 	Richard Pitman
> Sent: 	Monday, August 21, 2000 9:53 AM
> To: 	CJones6672@aol.com; histonet@pathology.swmed.edu
> Subject: 	Re: processor artifacts in tissue
> 
> 
> 
> Richard Pitman FIBMS,
> Head MLSO,
> Dept of Histology, Cytology & Immunology,
> Worcester Royal Infirmary NHS Trust
> 
> >>> <CJones6672@aol.com> 08/21/00 02:01am >>>
> My Histology Lab is currently using a Leica tissue processor and have 
> encountered some severe processing problems.  The stained slides have
> nuclear 
> "ghosting" and   a washed out appearance. The problem appears to be
> reagent 
> carry over as it usually starts occurring about 2 to 3 runs after all of
> the 
> reagents are changed. We have had the machine serviced and checked
> numerous 
> time without solving the problem. 
> Has anyone else encountered this problem with their Leica? 
> 
> In a word, yes.
> 
> It is obviously due to your junior staff not changing the solutions
> properly - well, that's what Leica told me ...
> 
> I have probably exhausted the patience of this list on this subject, so
> will e-mail privately. Previous postings should be in the archives, for
> the curious.
> 
> Good luck, 
> 
>