RE: kappa and lambda IHC

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From:"Drew Sally A." <>
To:'Special Histology Lab Tech' <>, 'Histonet' <>

We are using BioCare Medical's monoclonal kappa and lambda on our Ventana
genII and ES.  For formalin-fixed paraffin embedded tissues, pretreatment
includes using BioCare's ReVeal buffer for HIER in a pressure cooker.  For
B5-fixed, decal'd bone marrow biopsies we use 1mM EDTA, pH 8.0 for the
retrieval buffer.  We're much happier with our stains now than we were with
polyclonal antibodies.

Sally Ann Drew-MT(ASCP)
Univ. of Wis. Hospital and Clinics
IHC/ISH Clinical/Research Lab
Madison, WI 

> -----Original Message-----
> From:	Special Histology Lab Tech []
> Sent:	Monday, August 21, 2000 1:09 PM
> To:
> Subject:	kappa and lambda IHC
> To All:
> We have been having some problems with the consistency of staining 
> for  kappa and lambda on formalin fixed tissue. Just wondering if we 
> can get some feedback on which abs. and what kind of pretreatments 
> are being used. Thanks.
> Michele Margiotta
> Special Histology Lab
> UH @ Stony Brook, NY 

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