We are using BioCare Medical's monoclonal kappa and lambda on our Ventana
genII and ES.  For formalin-fixed paraffin embedded tissues, pretreatment
includes using BioCare's ReVeal buffer for HIER in a pressure cooker.  For
B5-fixed, decal'd bone marrow biopsies we use 1mM EDTA, pH 8.0 for the
retrieval buffer.  We're much happier with our stains now than we were with
polyclonal antibodies.

Sally Ann Drew-MT(ASCP)
Univ. of Wis. Hospital and Clinics
IHC/ISH Clinical/Research Lab
Madison, WI 
(608)265-6596
(608)263-1568(fax)

> -----Original Message-----
> From:	Special Histology Lab Tech [SMTP:spectech@path.som.sunysb.edu]
> Sent:	Monday, August 21, 2000 1:09 PM
> To:	histonet@pathology.swmed.edu
> Subject:	kappa and lambda IHC
> 
> To All:
> We have been having some problems with the consistency of staining 
> for  kappa and lambda on formalin fixed tissue. Just wondering if we 
> can get some feedback on which abs. and what kind of pretreatments 
> are being used. Thanks.
> 
> Michele Margiotta
> Special Histology Lab
> UH @ Stony Brook, NY