RE:freezing and sectioning Matrigel
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From: | Tamara Howard <howard@cshl.org> |
To: | Histology listserver <histonet@pathology.swmed.edu>, Emily.Yandl@genzyme.com |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
Emily -
I've never frozen the stuff, but I have worked with it for TEM and
it is *very* difficult to dehydrate - your investigator is going to have a
lot of ice crystals to deal with...probably why the morph is so bad. Have
you tried cryoprotecting the samples (with sucrose or something)? I don't
know if it would help, but it probably wouldn't hurt to try!
Good luck!
Tamara Howard
CSHL
>I'm wondering if anyone out there has experience working with a substance
>called Matrigel? An investigator here is trying to freeze blocks of this
>gel with cells in 24-well plates and is experiencing difficulties with
>morphology preservation (suprise!). If you have worked with gel, I would
>love advice on freezing and/or cryopreservation techniques.
>
>Thanks,
>
>Emily Ezernieks
>Genzyme Corp.
>emily.yandl@genzyme.com
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