RE: fishheads & boneheads - otolith aging

<< Previous Message | Next Message >>
From:Alan Bright <>,
Content-Type:text/plain; charset=iso-8859-1

Dear Carol,

Some years back we supplied a Bright 5040 microtome for sectioning
undecalcified otoliths in Cod, I can give you the  contact details in the UK
if this would help.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
PE18 6EB

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Web Site:

-----Original Message-----
From: []
Sent: 14 August 2000 12:54
Subject: fishheads & boneheads - otolith aging

Greetings Histonetters:

Has anyone done aging of fish on decalcified otoliths (earbones) embedded in
paraffin?  I am concerned that I won't see the growth rings after
decalcification.  If it can be done, how should the otolith be oriented for
sectioning?  Is H&E a good stain?  I have a fisheries biologist who used to
do histo thinking that this might be an easier method than grinding the
otoliths.  He wants to do this on striped bass (Morone saxatilis).

Thanks for your help.

Regards -
Carol B. McCollough, HT(ASCP)
Diagnostics & Histology Laboratory Manager
Maryland Department of Natural Resources
Fisheries Service
Cooperative Oxford Laboratory
904 S. Morris Street
Oxford, MD 21654

<< Previous Message | Next Message >>