RE: brazilin 2nd opinion
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From: | "Komuves, Laszlo" <LKOMUVES@corr.com> |
To: | "'J. A. Kiernan'" <jkiernan@julian.uwo.ca>, "Smith, Allen" <asmith@mail.barry.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=iso-8859-1 |
I do not think that Dr. Kiernan remembers our correspondence about brasilin
about five years ago, but it might be useful to recall it for people who are
trying to use brasilin.
I decided to try it for counterstaining ISH developed by BCIP/NBT substrates
(blue precipitate) since I found that Nuclear Fast Red did not give me a
strong nuclear counterstain.
I read about brasilin in Dr. Kiernan's book. I have the new edition.
Everybody doing histological stains should have this book. However,
witnessing the messages posted on Histonet, it seems that very few people
have time to actually read a texbook. (But that is a whole other story.)
I purchased brasilin from ICN, made the stain and it did not work. Contacted
Dr. Kiernan, who helped me to troubleshoot. Eventually I determined that the
lot I received did not have any active dye in it (due either to mislabel or
ageing/oxidation/improper storage, etc.)
I gave up on nuclear counterstaining since then and now I am using DIC
imaging to visualize tissue morphology.
I do not recommend wasting any money on brasilin until a company or the
Stain commision certifies a lot.
Sincerely,
László G. Kömüves, Ph.D.
Senior Scientist, Histology
COR Therapeutics, Inc.
256 East Grand Avenue
South San Francisco CA 94080
Phone: (650) 244-6855, Fax: (650) 244-9270
-----Original Message-----
From: J. A. Kiernan [mailto:jkiernan@julian.uwo.ca]
Sent: Monday, August 28, 2000 7:52 AM
To: Smith, Allen
Cc: Histonet
Subject: Re: brazilin
On Sun, 27 Aug 2000, Smith, Allen wrote:
> I used to use brazilin, but I have been unable to find it lately. Where
can
> I buy it?
Dear Allen, Your question may be of wider interest, so I'm
forwarding the answer to HistoNet, in the hope that someone
knows a less expensive supplier. I could find Brazilin in only
two catalogues. Details below.
I like alum-brazilin as a red nuclear stain, but the dyestuff
has become ridiculously expensive in the last 10 years or so.
I've still got a bit left over from the 1970s when its price
wasn't in any way remarkable.
Brazilin. CI 74280 Natural red 24
ICN Biomedicals Inc. Cat # 154862 US$58 for 1 gm.
VWR Canlab Rare & Fine Chemicals. Cat # 205613 US$1776.50 for 10 gm.
(VWR say they can supply smaller amounts!)
The 2nd of these catalogues is more recent (2000). It seems
to be an ICN catalog in a VWR wrapper.
Brazilwood chips can be bought for back-to-nature home dyeing.
Brazilin is obtained by evaporating an alcoholic extract, in
just the same way that haematoxylin is made from logwood.
Sounds easy if you have the time; I've never tried it myself.
Some years ago a Histonetter in Australia reported having
made some workable haematoxylin out of home-grown logwood,
and there was other correspondence about this and brazilin
around the same time. It should be in the Archives.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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