Try Prolong Antifade from Molecular Probes.  It's close to permanent.



Jennifer M. Philopena
Scientist 1
Canji, Inc.
3525 John Hopkins Court
San Diego, CA 92121
jennifer.philopena@canji.com

> ----------
> From: 	Lesley Weston
> Sent: 	Thursday, August 17, 2000 10:19 AM
> To: 	Gayle Callis
> Cc: 	histonet@pathology.swmed.edu
> Subject: 	Re: Permanent mounting media for AEC
> 
> Do you happen to know if these mounting media autofluoresce? (I don't know
> what
> AEC is, so this might be a silly question.) I've been searching for a
> permanent
> aqueous mdium for various fluorescent stains, so if one or more of the
> mountants
> you mention is free of fluorescence, then I would love to know about it.
> Thanks.
> 
> Lesley Weston.
> 
> 
> 
> On Sun, 6 Aug 2000, Gayle Callis wrote:
> 
> > You can still purchase Crystal Mount from Fisher aka Fisher Healthcare
> > catalog for clinical labs, still made by Biomeda Corp. Have used this
> with
> > great success for AEC and is available in larger volume (bulk) bottle
> and
> > sent with smaller dispensing bottle, far cheaper than individual tiny
> > bottle purchases.  
> > 
> > Seems this "permanent aqueous" mounting media is available from several
> > companies.  Have a sample from Biogenex which is excellent called
> > Supermount.  Dako also carries one in their catalog, name escapes me,
> and
> > the AquaPerm from Shandon Lipshaw.  They all work the same way.
> > 
> > 
> > 
> > 
> > 
> > 
> > Gayle Callis
> > Veterinary Molecular Biology
> > Montana State University
> > Bozeman MT 59717-3610
> > 406 994-4705
> > 406 994-4303
> > 
> > 
> 
>