I haven't seen that a little more or less of Tween makes any difference in
labeling. I pipet about 0.5 ml more than called for and figure the extra 0.5
stays in the pipet.

Tim Morken, BA, EMT(MSA), HTL(ASCP)
Infectious Disease Pathology Activity
Centers for Disease Control and Prevention
Ms-G32
1600 Clifton Road
Atlanta, GA 30333
USA

PH: 404-639-3964
FAX: 404-639-3043

email: tim9@cdc.gov
-----Original Message-----
From: Louise Taylor [mailto:louiset@mail.saimr.wits.ac.za]
Sent: Tuesday, August 29, 2000 3:54 AM
To: histonet
Subject: MEASURING ACCURATELY



Hello histonetters

I want to ask a REALLY DUMB question: How does one accurately measure out
Tween 20 ( pipette, measuring cylinder)? It is so  viscous that I always
doubt that i have washed out/expelled the correct amount.

Thanks
louise taylor


Research Laboratory
Department of Anatomical Pathology
South African Institute for Medical Research
Johannesburg
South Africa