RE: IP's with Decal

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From:"Morken, Tim" <>
To:'Histonet' <>
Content-Type:text/plain; charset=iso-8859-1

So how long does it take to decal with this mixture?

-----Original Message-----
From: Su, Phy-Huynh []
Sent: Tuesday, August 22, 2000 5:42 PM
To: 'Histonet'
Subject: RE: IP's with Decal

I am using EDTA buffered to decal, too.  I made it myself to have a higher
concentration of EDTA to decal faster.  Typically I'll have 12.% to 14% EDTA
in the solution.  Boil your water in a microwave and pour EDTA powder in.
That will dissolve EDTA very quickly.  Cool it down, then buffer it, and add
other stuff.  You can have your 4% paraformaldehyde, or 10% NBF in the
solution (if you fix your tissues using those) to fix and decal at the same
time.  I also have some MgCl2 in my decal solution.  (Only add MgCl2 after
the solution was buffered.)  This seems to help to keep my Safranin-O
staining for proteoglycan good.  

Other people decal at 37 degree, or using microwave.  This seems to speed up
decal time.  But I have lost some antigenicity this way, so I prefer to do
it at RT or at 4 degree with stirring.
If you want to try my recipe, e-mail me.
Good luck

> -----Original Message-----
> From:	Abizar Lakdawalla []
> Sent:	Tuesday, August 22, 2000 1:07 PM
> To:	Jennifer Englin
> Cc:
> Subject:	Re: IP's with Decal
> EDTA (versene) based decal solutions work best for most immuno's.
> Downside: it's expensive and takes much more longer to decal.
> abizar
> 1-877IGX-INFO
> Jennifer Englin wrote:
> > Has anyone found a decal that works well with immuno's?
> > We are currently using Stephens Scientific Decal- formic
> acid/formaldahyde/mathanol, and we are having trouble with our Kappa and
> Lambda.
> > Any suggestions?
> >
> > Jennifer,
> > Willmar MN

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