I am using EDTA buffered to decal, too.  I made it myself to have a higher
concentration of EDTA to decal faster.  Typically I'll have 12.% to 14% EDTA
in the solution.  Boil your water in a microwave and pour EDTA powder in.
That will dissolve EDTA very quickly.  Cool it down, then buffer it, and add
other stuff.  You can have your 4% paraformaldehyde, or 10% NBF in the
solution (if you fix your tissues using those) to fix and decal at the same
time.  I also have some MgCl2 in my decal solution.  (Only add MgCl2 after
the solution was buffered.)  This seems to help to keep my Safranin-O
staining for proteoglycan good.  

Other people decal at 37 degree, or using microwave.  This seems to speed up
decal time.  But I have lost some antigenicity this way, so I prefer to do
it at RT or at 4 degree with stirring.
 
If you want to try my recipe, e-mail me.
Good luck
Su


> -----Original Message-----
> From:	Abizar Lakdawalla [SMTP:abizarl@innogenex.com]
> Sent:	Tuesday, August 22, 2000 1:07 PM
> To:	Jennifer Englin
> Cc:	histonet@pathology.swmed.edu
> Subject:	Re: IP's with Decal
> 
> EDTA (versene) based decal solutions work best for most immuno's.
> Downside: it's expensive and takes much more longer to decal.
> abizar
> www.innogenex.com
> 1-877IGX-INFO
> 
> Jennifer Englin wrote:
> 
> > Has anyone found a decal that works well with immuno's?
> > We are currently using Stephens Scientific Decal- formic
> acid/formaldahyde/mathanol, and we are having trouble with our Kappa and
> Lambda.
> > Any suggestions?
> >
> > Jennifer,
> > Willmar MN
>