RE: IHC controls
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From: | "Drew Sally A." <sa.drew@hosp.wisc.edu> |
To: | 'Lorraine C Smallwood' <lsmallwo@juno.com>, 'Histonet' <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain |
We cut our controls in advance for those markers, and after drying welll
overnight we store them in -20 degree C freezer. We only cut maybe 2
month's worth at a time.
Sally Ann Drew-MT(ASCP)
Univ. of Wis. Hospital and Clinics
IHC/ISH Clinical/Research Lab
Madison, WI
(608)265-6596
(608)263-1568(fax)
> -----Original Message-----
> From: Lorraine C Smallwood [SMTP:lsmallwo@juno.com]
> Sent: Friday, August 18, 2000 10:45 AM
> To: Histonet@pathology.swmed.edu
> Subject: IHC controls
>
> I need some advice on an issue that we are discussing in our lab and that
> is the issue of IHC controls, i.e.ER, PR, c-erB-2 (or her-2-neu). What
> are other labs doing as far as cutting controls for testing of these
> antibodies? Our pathologist wants us to "cut" controls as needed and and
> seal the block each time we use the block. He does not want "pre-cut"
> controls because of concerns of oxidation of the antigenic sites, hence,
> subsequent loss of reactivity. I'm just curious what other labs are
> doing.
> Thanks in advance!
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