RE: IHC controls

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From:"Drew Sally A." <>
To:'Lorraine C Smallwood' <>, 'Histonet' <>

We cut our controls in advance for those markers, and after drying welll
overnight we store them in -20 degree C freezer.  We only cut maybe 2
month's worth at a time.

Sally Ann Drew-MT(ASCP)
Univ. of Wis. Hospital and Clinics
IHC/ISH Clinical/Research Lab
Madison, WI 

> -----Original Message-----
> From:	Lorraine C Smallwood []
> Sent:	Friday, August 18, 2000 10:45 AM
> To:
> Subject:	IHC controls
> I need some advice on an issue that we are discussing in our lab and that
> is the issue of IHC controls, i.e.ER, PR, c-erB-2 (or her-2-neu).  What
> are other labs doing as far as cutting  controls for testing of these
> antibodies?  Our pathologist wants us to "cut" controls as needed and and
> seal the block each time we use the block.  He does not want "pre-cut"
> controls because of concerns of oxidation of the antigenic sites, hence,
> subsequent loss of reactivity.  I'm just curious what other labs are
> doing. 
> Thanks in advance!
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