RE: Fontana-Masson staining on appendix
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From: | "MacDonald, Jennifer" <jmacdonald@sach.org> |
To: | "\"Histonet@pathology.swmed.edu\" " <Histonet@pathology.swmed.edu>, "'CHEN, YUETIAN'" <YUETIAN.CHEN@spcorp.com> |
Reply-To: | |
Content-Type: | text/plain |
We routinely do this stain on paraffin emedded material.
> ----------
> From: CHEN, YUETIAN[SMTP:YUETIAN.CHEN@spcorp.com]
> Sent: Wednesday, August 16, 2000 2:20 PM
> To: "Histonet@pathology.swmed.edu"
> Subject: Fontana-Masson staining on appendix
>
>
> Dear histonetters:
> I am taking htl exam and on of the special staining is Fontana-Masson
> staining
> on appendix. According to Carson's book, alcohol should be avoided
> because
> alcohol dissolves argentaffin granules. Does that mean I need to do the
> staining
> on frozen section? Actually, in the procedure, it indicates the staining
> can be
> done on paraffin section because first step is deparaffinize. After
> stianing,
> the section can be dehydrate and clear with alcohol. I am confused.
> Should
> I
> use frozen tissues for my exam and totally avoid alcohol from begining to
> the
> end of the staining? Since finding the tissue is not easy for me, I need
> to
>
> make sure I am doing it right. Would you be able to give me some
> suggestions?
> Thank you very much.
> Yuetian
>
>
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