RE: Fontana-Masson staining on appendix

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From:"MacDonald, Jennifer" <jmacdonald@sach.org>
To:"\"Histonet@pathology.swmed.edu\" " <Histonet@pathology.swmed.edu>, "'CHEN, YUETIAN'" <YUETIAN.CHEN@spcorp.com>
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Content-Type:text/plain

We routinely do this stain on paraffin emedded material.

> ----------
> From: 	CHEN, YUETIAN[SMTP:YUETIAN.CHEN@spcorp.com]
> Sent: 	Wednesday, August 16, 2000 2:20 PM
> To: 	"Histonet@pathology.swmed.edu" 
> Subject: 	Fontana-Masson staining on appendix
> 
>      
> Dear histonetters:
> I am taking htl exam and on of the special staining is Fontana-Masson
> staining 
> on appendix.  According to Carson's book, alcohol should be avoided
> because 
> alcohol dissolves argentaffin granules. Does that mean I need to do the
> staining
> on frozen section?  Actually, in the procedure, it indicates the staining
> can be
> done on paraffin section because first step is deparaffinize.  After
> stianing, 
> the section can be dehydrate and clear with alcohol.  I am confused.
> Should
> I 
> use frozen tissues for my exam and totally avoid alcohol from begining to
> the 
> end of the staining?  Since finding the tissue is not easy for me, I need
> to
> 
> make sure I am doing it right.  Would you be able to give me some
> suggestions?  
> Thank you very much.
> Yuetian
> 
> 



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