Yes.  It has been my experience with the Ventana ES that they do not stain
well after spending the night in water or buffer.

Tom Mc Nemar
Pathology Supervisor
Licking Memorial Hospital
Newark, Ohio

> -----Original Message-----
> From:	Louise Taylor [SMTP:louiset@mail.saimr.wits.ac.za]
> Sent:	Tuesday, August 29, 2000 6:43 AM
> To:	histonet
> Subject:	Dewaxing slides
> 
> Hi histonetters
> 
> Has anyone had any experience with loss of antigenicity in slides that
> have
> been dewaxed and either left soaking in water/buffer overnight or allowed
> to
> airdry?
> 
> The reason I ask this is that we are moving to an automated IHC sytem in
> the
> near future and will be dealing with a lot of slides and a short turn
> around
> time.
> 
> Any input appreciated
> 
> Louise Taylor
> Research Laboratory
> Department of Anatomical Pathology
> South African Institute for Medical Research
> Johannesburg
> South Africa
> 
> 
>