"Due to circumstances beyond our control) we have, on rare occasions taken
paraffin sections all the way through retrieval methods and left them in
buffer overnight without a problem.  With some antibodies(like ER/PR) I feel
safer leaving the retrieval until the next morning, but we've not done a
study to see if there are any detrimental effects to doing this.

Sally Ann Drew-MT(ASCP)
Univ. of Wis. Hospital and Clinics
IHC/ISH Clinical/Research Lab
Madison, WI 
(608)265-6596
(608)263-1568(fax)

> -----Original Message-----
> From:	Louise Taylor [SMTP:louiset@mail.saimr.wits.ac.za]
> Sent:	Tuesday, August 29, 2000 5:43 AM
> To:	histonet
> Subject:	Dewaxing slides
> 
> Hi histonetters
> 
> Has anyone had any experience with loss of antigenicity in slides that
> have
> been dewaxed and either left soaking in water/buffer overnight or allowed
> to
> airdry?
> 
> The reason I ask this is that we are moving to an automated IHC sytem in
> the
> near future and will be dealing with a lot of slides and a short turn
> around
> time.
> 
> Any input appreciated
> 
> Louise Taylor
> Research Laboratory
> Department of Anatomical Pathology
> South African Institute for Medical Research
> Johannesburg
> South Africa
> 
> 
>