Why frozen section, if the required thickness is in fact 300 micrometers?
Fresh, untreated soft tissue can be cut using a Vibratome from 20 micrometers 
up. Above 150 micrometers they can also be sectioned by a simpler instrument, a 
tissue chopper. Incidentally, I micrometer sections of fresh tissues are 
possible with tissue and brain matrixes.
Disclaimer: ProSciTech supplies these instruments, so we have a vested 
interest. Read our files on these if interested, but note that we supply these 
instruments anywhere but not into N America.
Cheers
Jim Darley
ProSciTech                 Microscopy PLUS
PO Box 111, Thuringowa  QLD  4817  Australia
Ph +61 7 4774 0370  Fax:+61 7 4789 2313  service@proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560                      www.proscitech.com

On Wednesday, August 16, 2000 7:58 AM, Karen Pawlowski 
[SMTP:karen.pawlowski@worldnet.att.net] wrote:
> Hi Cindy,
>
> You could use a sliding microtome with crushed dry ice around the sample
> to cut
> a 300 micron section.  That's the only way I've seen it done.
>
> Karen
>
> DELONG_CYNTHIA_A@LILLY.COM wrote:
>
> > Hello,
> >
> > I would like to know what piece of equipment would cut 300 mm frozen
> > sections.  I have a post doc that needs to do this procedure.
> >
> > Thank you
> > Cindy
>
#000##000#