PNA staining

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From:Kim Kusser <kkusser@trudeauinstitute.org>
To:histonet@pathology.swmed.edu
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Hi all,

I've been trying to reduce the non-specific background staining I get when I use PNA (biotinylated). I block with 5% BSA (more than enough), and Avidin/Biotin block (vector kit). I've titrated the dilution as high as I can without losing the specific staining that I want. Problem is...PNA is one sticky lectin. Either that and/or it has other things it likes to stick to besides the germinal centers...like epithelium.

BTW, I stain frozen mouse sections...spleen, NALT, Peyer's patches, Lymph nodes and lung.

All tips and tricks appreciated

Kim



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