Methacarn fixative

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From:"Philopena, Jennifer" <>
To:'Histonet' <>
Content-Type:text/plain; charset=windows-1252

I've read that Methacarn is a methanol-Carnoy's fixative, Carnoy's
consisting of 6 parts ethanol, 3 parts chloroform, and one part glacial
acetic acid.  In methacarn does the methanol replace the ethanol?  Does
anyone have any more information on this fix?  History, recipe,
advantages/disadvantages, will using it as a fixative eliminate the need for
antigen retrieval (as I've seen in the literature)?  Thanks.  Jen

Jennifer M. Philopena
Scientist 1
Canji, Inc.
3525 John Hopkins Court
San Diego, CA 92121

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