MMA sectioning and using slide warmer
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From: | Nancy Maronto <nmaronto@hotmail.com> |
To: | HOOVER_JENNIFER@LILLY.COM |
Reply-To: | |
Content-Type: | text/plain; format=flowed |
Jennifer,
I use a slide warmer for thin cut sections at approximately 37 degrees C.
Usually wet cutting the block with a lower % alcohol then place the tissue
on a warm treated slide with 70% alcohol. work out the wrinkles gently with
forceps, then I place plastic on the section roll with a small roller to
flatten and remove to a table. Some plastics will wrinkle as they set on
the slide warmer. changing the % alcohol on the block face or on the slide
may also prove effective.
Hope this helps.
Nancy Maronto
MPI Research
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