IP's with Decal

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From:Gayle Callis <uvsgc@msu.oscs.montana.edu>
To:Abizar Lakdawalla <abizarl@innogenex.com>, histonet@pathology.swmed.edu
Content-Type:text/plain; charset=us-ascii

Formalin continues to crosslink as you are decalcifying, it may be better
to fix with formalin, THEN decalcify with a buffered formic acid solution,
approx 4.5% formic acid with either sodium formate or sodium citrate.
Antigen retrieval would still have to be done.  Some of the commercially
available formic acid/formalin mixtures contain 10% formic acid, and if the
rate of decalcification exceeds the rate of fixation, antigens may well be
damaged by the acid BEFORE they are fixed.  Usually these combo fixatives
are great for small biopsies, but possibly not so good for immunostaining.

Try fixation first with possibly less time, then decalcify, retrieve or
enzyme treatment whatever works best and stain (saw a lot of discussion on
Kappa and Lambda this past week).  You may get better results.

EDTA is ideal for immunohistochemistry, but most clinical setting opt for
speed, etc.

Just some suggestions and thoughts.

t 10:07 AM 8/22/00 -0700, you wrote:
>EDTA (versene) based decal solutions work best for most immuno's.
Downside: it's expensive and takes much more longer to decal.
>Jennifer Englin wrote:
>> Has anyone found a decal that works well with immuno's?
>> We are currently using Stephens Scientific Decal- formic
acid/formaldahyde/mathanol, and we are having trouble with our Kappa and
>> Any suggestions?
>> Jennifer,
>> Willmar MN
Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303

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