IHC controls

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From:Lorraine C Smallwood <lsmallwo@juno.com>
To:Histonet@pathology.swmed.edu
Reply-To:
Content-Type:text/plain

I need some advice on an issue that we are discussing in our lab and that
is the issue of IHC controls, i.e.ER, PR, c-erB-2 (or her-2-neu).  What
are other labs doing as far as cutting  controls for testing of these
antibodies?  Our pathologist wants us to "cut" controls as needed and and
seal the block each time we use the block.  He does not want "pre-cut"
controls because of concerns of oxidation of the antigenic sites, hence,
subsequent loss of reactivity.  I'm just curious what other labs are
doing. 
Thanks in advance!
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