Fw: IHC controls

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From:Jim Manavis <jim.manavis@imvs.sa.gov.au>
To:Histonet <HistoNet@Pathology.swmed.edu>
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The concern most people have is the oxidation of antigenic sites over time
and loss of immunoreactivity. What I do and have found this to preserve
staining is to store cut control slides at -4 degrees. I store controls for
ER, PgR, CD5, MLH1, MSH2 all in the freezer.

Jim Manavis
-----Original Message-----
From: Lorraine C Smallwood <lsmallwo@juno.com>
To: Histonet@pathology.swmed.edu <Histonet@pathology.swmed.edu>
Date: Saturday, 19 August 2000 12:09
Subject: IHC controls

>I need some advice on an issue that we are discussing in our lab and that
>is the issue of IHC controls, i.e.ER, PR, c-erB-2 (or her-2-neu).  What
>are other labs doing as far as cutting  controls for testing of these
>antibodies?  Our pathologist wants us to "cut" controls as needed and and
>seal the block each time we use the block.  He does not want "pre-cut"
>controls because of concerns of oxidation of the antigenic sites, hence,
>subsequent loss of reactivity.  I'm just curious what other labs are
>Thanks in advance!
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