Frozen tissues

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From:Connie McManus <>
To:Histonet <>
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Greetings everyone!

I just got a call from the guy I do HBV IHC for and he has a project for
me.  Th problem is that all his tissues (transgenic mouse livers) are
frozen, the tech who froze them put them in lq N2 but DID NOT use a
cryopreservant.  We want to do frozen sections on these and perform HBV
IHC on them.  Is it best to defrost them and fix in BNF or leave them
frozen?  The concern here is all the other work I've done has been on
BNF fixed & paraffin embedded tissues and we would like some modicum of


Connie McManus

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