Fontana-Masson staining on appendix

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From:"CHEN, YUETIAN" <YUETIAN.CHEN@spcorp.com>
To:"\"Histonet@pathology.swmed.edu\" " <Histonet@pathology.swmed.edu>
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Dear histonetters:
I am taking htl exam and on of the special staining is Fontana-Masson
staining 
on appendix.  According to Carson's book, alcohol should be avoided because 
alcohol dissolves argentaffin granules. Does that mean I need to do the
staining
on frozen section?  Actually, in the procedure, it indicates the staining
can be
done on paraffin section because first step is deparaffinize.  After
stianing, 
the section can be dehydrate and clear with alcohol.  I am confused.  Should
I 
use frozen tissues for my exam and totally avoid alcohol from begining to
the 
end of the staining?  Since finding the tissue is not easy for me, I need to

make sure I am doing it right.  Would you be able to give me some
suggestions?  
Thank you very much.
Yuetian




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