Diffquik/Toludine Blue Methodology

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Several people have asked me questions about the Diff-Quik II stain for 
Helicobacter pylori.

I have a detailed method, in NCCLS format with references, on my Web site at 

I have introduced this method in at least five laboratories in my travels. In 
my personal opinion it is quite as good as the more complex methods.

I have a procedure for the Genta stain, but have never even seen the stain 
for myself. I think several people on this list perform it. The reference is
Robert M. Genta MD. George O. Robason HT(ASCP). David Y. Graham MD. (Baylor 
in Houston, at VA center there). Simultaneous visualization of Helicobacter 
pylori and gastric morphology: a new stain.
Human Pathology, March 1994: 25;221-6.

Bob Richmond
Samurai Pathologist
Knoxville TN

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