Hi histonetters

Has anyone had any experience with loss of antigenicity in slides that have
been dewaxed and either left soaking in water/buffer overnight or allowed to
airdry?

The reason I ask this is that we are moving to an automated IHC sytem in the
near future and will be dealing with a lot of slides and a short turn around
time.

Any input appreciated

Louise Taylor
Research Laboratory
Department of Anatomical Pathology
South African Institute for Medical Research
Johannesburg
South Africa