methyl-green staining or ethyl-green staining

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From:Peter Poon <>
To:histonet <>
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Oka.  we are currently trying to use ethyl-green as a nuclear counter
stain to a immunohistostain for Estrogen receptor.  Most of the slides we
do the background (neuropil) shows ups as pale blue in contrast to the
nucleus, which is darker blue, but there are a few random slide in which
the ethyl-green looks perfect, it stains the nucleus, but leaves the
background unstained.  Does anyone know what happened? And how this can be
duplicated. I'm at my wit's end on this one.

Peter Poon, histology rookie.

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